Searchable abstracts of presentations at key conferences in endocrinology

ea0029p891 | Female Reproduction | ICEECE2012

Androgen profiling in adolescent females by liquid chromatography–tandem mass spectrometry

Fanelli F. , Belluomo I. , Di Lallo V. , Prontera O. , Repaci A. , Di Dalmazi G. , Pagotto U. , Gambineri A. , Pasquali R.

Polycystic ovary syndrome (PCOS) has an early onset. However, its prevalence in the youngest age groups has not been assessed, mostly due to the poor specificity of the criteria for defining PCOS in adolescents, including the testosterone references generated only on adult women. Moreover, the current references for testosterone were mostly built by immunoassays, known to have poor accuracy. This study was aimed at defining androgen and pro-androgen reference intervals in 149 ...

ea0026p13 | Adrenal cortex | ECE2011

Redefinition of age and sex specific reference intervals of eight serum steroids by liquid chromatography: tandem mass spectrometry (LC–MS/MS)

Fanelli F , Belluomo I , Di Lallo V D , Baccini M , Casadio E , Gasparini D Ibarra , Gambineri A , Pasquali R , Pagotto Uberto

The use of LC–MS/MS quantitative methods in clinical and research laboratories is becoming a well established reality that may overcome specificity and accuracy limits of immunoassays. Nevertheless, among different LC–MS/MS methods an agreement on the values that are to be considered normal or pathologic has not been reached yet, and this is mainly due to differences in LC–MS/MS methods, to the poor or absent availability of reference material and to the selecti...

ea0029oc12.1 | Obesity Clinical | ICEECE2012

Circulating endocannabinoids are associated to cardiometabolic alterations independently from adiposity

Fanelli F. , Mezzullo M. , Belluomo I. , Di Lallo V. , Colavita M. , Baccini M. , Casadio E. , Ibarra Gasparini D. , Vicennati V. , Pasquali R. , Pagotto U.

Obesity is characterized by an increase of endocannabinoid circulating levels as anandamide (AEA) and 2-arachidonoyl-glycerol (2AG). Whether these lipids represent a biomarker of dysmetabolism or merely reflect the fat mass spill-over is still unclear. After generating rigorous, gender specific, plasma reference intervals by 2D-liquid chromatography-tandem mass spectrometry, we defined the 97.5thcentile of endocannabinoid distribution in the normal population as the cut-off ab...

ea0026p330 | Obesity | ECE2011

Haematic circulating endocannabinoids (ECs) in lean, overweight and obese: the role of the gender

Fanelli F , Di Lallo V D , Belluomo I , Casadio E , Baccini M , Ibarra Gasparini D , Vicennati V , Pasquali R , Pagotto Uberto

A number of publications recently provided measurements of plasma ECs by liquid chromatography–tandem mass spectrometry (LC–MS/MS), however the reported circulating levels in normal subjects are scattered in a very wide range of concentrations. A quantitative LC–MS/MS method for anandamide (AEA), palmitoylethanolamide (PEA), oleoylethanolamyde (OEA) and 2arachidonoyl-glycerol (2AG), chromatographically resolved by its inactive isomer 1AG, was validated in house....